Search
Invitrogen
PIK3CA Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 5
PIK3CA Antibody (PA5-88576) in IHC (P)
Immunohistochemistry analysis of PIK3CA in paraffin-embedded human breast cancer using. Samples were incubated with PIK3CA Polyclonal antibody (Product # PA5-88576) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-88576
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PIK3CA (NP_0062092)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.34 mg/mL
Amount
34 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse kidney
Immunogen sequence: DCFTMPSYSR RISTATPYMN GETSTKSLWV INSALRIKIL CATYVNVNIR DIDKIYVRTG IYHGGEPLCD NVNTQRVPCS NPRWNEWLNY DIYIPDLPRA ARLCLSICSV KGRKGAKEEH CPLAWGNINL FDYTDTLVSG KMALNLWPVP HGLEDLLNPI GVTGSNPNKE TPCLELEFDW FSSVVKFPDM SVIEEHANWS VSREAGFSYS HAGLSNRLAR DNELRENDKE QLKAISTRDP LSEITEQEKD FLWSHRHYCV TIPEILPKLL LSVKWNSRDE VAQMYCLVKD WPPIKPEQAM E
Target Information
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 2.7.1.153; EC:2.7.11.1; GRB1; MGC142161; MGC142163; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphoinositide-3-kinase catalytic alpha polypeptide; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3-kinase subunit alpha; PI3K alpha; PI3K-alpha; PI3Kalpha; pi3kca; PIK3R1; pk3ca; ptdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; Serine/threonine protein kinase PIK3CA
Gene Aliases: 6330412C24Rik; caPI3K; p110; p110alpha; Pik3ca
UniProt ID: (Mouse) P42337, (Rat) A0A0G2K344
Entrez Gene ID: (Mouse) 18706, (Rat) 170911
nucleotide binding
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
1-phosphatidylinositol-3-kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
protein kinase activator activity
1-phosphatidylinositol-4-phosphate 3-kinase activity
insulin receptor substrate binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
kinase
angiogenesis
liver development
adaptive immune response
glucose metabolic process
protein phosphorylation
inflammatory response
phosphatidylinositol 3-kinase signaling
phosphorylation
platelet activation
positive regulation of peptidyl-serine phosphorylation
phosphatidylinositol-3-phosphate biosynthetic process
regulation of multicellular organism growth
regulation of cellular respiration
protein kinase B signaling
negative regulation of neuron apoptotic process
hypomethylation of CpG island
innate immune response
positive regulation of protein kinase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
cell chemotaxis
adipose tissue development
cellular response to glucose stimulus
energy homeostasis
mitophagy in response to mitochondrial depolarization
negative regulation of fibroblast apoptotic process
regulation of genetic imprinting
negative regulation of anoikis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support