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Invitrogen
NDUFAF1 Polyclonal Antibody
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FIGURE: 1 / 3
NDUFAF1 Antibody (PA5-90745) in WB
Western Blot was performed using Anti-NDUFAF1 Polyclonal Antibody (Product # PA5-90745) and a 35 kDa band corresponding to Complex I intermediate-associated protein 30, mitochondrial was observed. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), SK-BR-3 (Lane 3), Jurkat (Lane 4), and MCF7 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-90745
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_0570972)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
3.78 mg/mL
Amount
378 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MALVHKLLRG TYFLRKFSKP TSALYPFLGI RFAEYSSSLQ KPVASPGKAS SQRKTEGDLQ GDHQKEVALD ITSSEEKPDV SFDKAIRDEA IYHFRLLKDE IVDHWRGPEG HPLHEVLLEQ AKVVWQFRGK EDLDKWTVTS DKTIGGRSEV FLKMGKNNQS ALLYGTLSSE APQDGESTRS GYCAMISRIP RGAFERKMSY DWSQFNTLYL RVRGDGRPWM VNIKEDTDFF QRTNQMYSYF MFTRGGPYWQ EVKIPFSKFF FSNRGRIRDV QHELPLDKIS SIGFTLADKV DGPFFLEIDF IGVFTDPAHT EEFAYENSPE LNPRLFK; Positive Samples: HeLa, 293T, A-549, Mouse heart, Rat liver; Cellular Location: Mitochondrion
Target Information
The NDUFAF1 gene encodes the human homolog of a Neurospora crassa Cia30 protein involved in the assembly of complex I.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1; NADH dehydrogenase (ubiquinone) 1 assembly factor 1; NADH dehydrogenase (ubiquinone) complex I, assembly factor 1; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, assembly factor 1; unnamed protein product
Gene Aliases: 2410001M24Rik; CGI-65; CGI65; CIA30; MC1DN11
UniProt ID: (Human) Q9Y375, (Mouse) Q9CWX2
Entrez Gene ID: (Human) 51103, (Mouse) 69702, (Rat) 296086
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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