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Invitrogen

WNT3A Polyclonal Antibody

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WNT3A Polyclonal Antibody

Product Details

PA5-95129

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Wnt3a (252-267aa WVETLRPRYTYFKVPT).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806934

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: SMMC whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC119418; MGC119419; MGC119420; unnamed protein product; vestigial tail; wingless-related MMTV integration site 3A; wingless-type MMTV integration site family, member 3A; Wnt 3a; WNT3A protein

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Gene Aliases: vt; Wnt-3a

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UniProt ID: (Human) P56704, (Mouse) P27467

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Entrez Gene ID: (Human) 89780, (Mouse) 22416

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Function(s)
transcription coactivator activity receptor binding frizzled binding cytokine activity protein binding protein domain specific binding co-receptor binding identical protein binding receptor agonist activity
Process(es)
positive regulation of receptor internalization heart development COP9 signalosome assembly gene expression regulation of gene expression positive regulation of gene expression negative regulation of neuron projection development Wnt signaling pathway cell proliferation in forebrain neurogenesis neuron differentiation midbrain development cell proliferation in midbrain non-canonical Wnt signaling pathway synaptic vesicle recycling cell fate commitment positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of mesodermal cell fate specification animal organ development positive regulation of skeletal muscle tissue development negative regulation of neurogenesis modulation of synaptic transmission regulation of synapse organization canonical Wnt signaling pathway cardiac muscle cell fate commitment positive regulation of dermatome development secondary palate development cellular response to retinoic acid positive regulation of canonical Wnt signaling pathway calcium ion transmembrane transport via low voltage-gated calcium channel presynapse assembly positive regulation of protein localization to plasma membrane negative regulation of dopaminergic neuron differentiation midbrain dopaminergic neuron differentiation regulation of postsynapse to nucleus signaling pathway regulation of presynapse assembly positive regulation of cardiac muscle cell differentiation osteoblast differentiation in utero embryonic development somitogenesis positive regulation of cytokine production heart looping signal transduction cell-cell signaling determination of left/right symmetry axonogenesis axon guidance mesoderm development protein localization positive regulation of cell proliferation organ morphogenesis anterior/posterior pattern specification spinal cord association neuron differentiation hippocampus development dorsal/ventral neural tube patterning hemopoiesis platelet activation extracellular matrix organization BMP signaling pathway mammary gland development positive regulation of B cell proliferation positive regulation of telomere maintenance via telomerase skeletal muscle cell differentiation post-anal tail morphogenesis inner ear morphogenesis regulation of cell differentiation negative regulation of fat cell differentiation somatic stem cell division paraxial mesodermal cell fate commitment positive regulation of collateral sprouting in absence of injury negative regulation of axon extension involved in axon guidance regulation of axonogenesis positive regulation of protein tyrosine kinase activity regulation of microtubule cytoskeleton organization platelet aggregation dopaminergic neuron differentiation axis elongation involved in somitogenesis positive regulation of cell-cell adhesion mediated by cadherin positive regulation of neural precursor cell proliferation positive regulation of hepatocyte proliferation regulation of RNA biosynthetic process
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