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Invitrogen
PP2A alpha Polyclonal Antibody
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FIGURE: 1 / 8
PP2A alpha Antibody (PA5-95235) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of PP2A alpha in U2OS cells using PP2A alpha Polyclonal Antibody (Product # PA5-95235). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 d... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95235
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell, human Hela whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human colorectal adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: U2OS cell. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B55-GAMMA; IMYPNO; IMYPNO1; Methyl-PPP2CA; MGC33570; phosphatase 2A; pp2A; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; PP2A-alpha; PPP2CA; PR52; PR55G; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic alpha subunit; protein phosphatase-2A-alpha; replication protein C; type-2A protein phosphatase catalytic subunit; unnamed protein product
Gene Aliases: HJS3; NEDLBA; PP2A; Pp2a1; PP2Ac; PP2CA; PP2Calpha; RP-C
UniProt ID: (Human) P67775, (Mouse) P63330, (Rat) P63331
Entrez Gene ID: (Human) 5515, (Mouse) 19052, (Rat) 24672
phosphoprotein phosphatase activity
protein serine/threonine phosphatase activity
protein tyrosine phosphatase activity
protein binding
hydrolase activity
protein heterodimerization activity
tau protein binding
GABA receptor binding
RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity
RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity
RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity
enzyme binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
beta-2 adrenergic receptor binding
identical protein binding
protein kinase B binding
ion channel binding
macromolecular complex binding
protein phosphatase 2A binding
protein antigen binding
mitotic cell cycle
regulation of transcription from RNA polymerase II promoter
protein dephosphorylation
response to lead ion
negative regulation of epithelial to mesenchymal transition
snRNA processing
regulation of microtubule polymerization
regulation of transcription elongation from RNA polymerase II promoter
regulation of hippo signaling
negative regulation of hippo signaling
intracellular signal transduction
regulation of growth
T cell homeostasis
regulation of cell differentiation
negative regulation of protein kinase B signaling
negative regulation of canonical Wnt signaling pathway
vascular endothelial cell response to oscillatory fluid shear stress
RNA polymerase II transcription initiation surveillance
positive regulation of NLRP3 inflammasome complex assembly
negative regulation of glycolytic process through fructose-6-phosphate
regulation of G1/S transition of mitotic cell cycle
regulation of protein phosphorylation
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
mesoderm development
regulation of receptor activity
regulation of protein autophosphorylation
peptidyl-threonine dephosphorylation
regulation of protein catabolic process
positive regulation of glycolytic process
regulation of cell cycle
negative regulation of protein phosphorylation
cardiac ventricle development
heart development
cellular response to insulin stimulus
positive regulation of dephosphorylation
negative regulation of protein import into nucleus
cellular response to calcium ion
cellular response to glucose stimulus
cellular response to cytokine stimulus
cellular response to ethanol
cellular response to follicle-stimulating hormone stimulus
cellular response to steroid hormone stimulus
negative regulation of calcium ion transmembrane transporter activity
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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