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Invitrogen
Galectin 1 Polyclonal Antibody
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FIGURE: 1 / 6
Galectin 1 Antibody (PA5-95333) in ICC/IF
Immunocytochemistry analysis of Galectin 1 using anti-Galectin 1 antibody (Product # PA5-95333) . Galectin 1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Galectin 1 antibody (Product # PA5-95333) overnight at 4°C. DyLight®488 Conjuga... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95333
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Galectin 1 recombinant protein (Position: A2-D135).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human Galectin 1 shares 88% and 90% amino acid (aa) sequence identity with mouse and rat Galectin 1, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human A549 whole cell, human A375 whole cell, human MCF-7 whole cell, rat kidney tissue, rat PC-12 whole cell, mouse kidney tissue, mouse NIH/3T3 whole cell. IHC: mouse intestine cancer tissue, rat kidney tissue, human placenta tissue. ICC/IF: U20S cell. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14 kDa beta-galactoside-binding lectin (L-14.5); 14 kDa laminin-binding protein; 14 kDa lectin; beta-galactoside binding protein; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein; beta-galactoside-binding protein 14kDa; DKFZp686E23103; epididymis secretory sperm binding protein; gal-1; galaptin; Galectin1; H-Galectin-1; HBL; HLBP14; HPL; lactose-binding lectin 1; lectin galactoside-binding soluble 1; lectin precursor; lectin, galactose binding, soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; pm12; putative MAPK-activating protein PM12; RL 14.5; S-Lac lectin 1; unnamed protein product
Gene Aliases: Gal-1; GAL1; Galbp; galectin-1; GBP; L-14.5; L14; Lect14
UniProt ID: (Human) P09382, (Rat) P11762, (Mouse) P16045
Entrez Gene ID: (Human) 3956, (Rat) 56646, (Mouse) 16852
plasma cell differentiation
signal transduction
response to xenobiotic stimulus
negative regulation of cell-substrate adhesion
negative regulation of neuron projection development
T cell costimulation
positive regulation of erythrocyte aggregation
response to isolation stress
positive regulation of apoptotic process
maintenance of protein location
myoblast differentiation
positive regulation of viral entry into host cell
response to axon injury
positive regulation of inflammatory response
cellular response to glucose stimulus
cell-cell adhesion
negative regulation of T-helper 17 cell lineage commitment
positive regulation of dendritic cell differentiation
apoptotic process
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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