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Invitrogen
Cyclin D2 Polyclonal Antibody
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FIGURE: 1 / 2
Cyclin D2 Antibody (PA5-95922) in WB
Western blot analysis of Cyclin D2 in extracts of various cell lines. Samples were incubated with Cyclin D2 Polyclonal antibody (Product # PA5-95922) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95922
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human Cyclin D2 (NP_0017501)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
3.11 mg/mL
Amount
311 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MELLCHEVDP VRRAVRDRNL LRDDRVLQNL LTIEERYLPQ CSYFKCVQKD IQPYMRRMVA TWMLEVCEEQ KCEEEVFPLA MNYLDRFLAG VPTPKSHLQL LGAVCMFLAS KLKETSPLTA EKLCIYTDNS IKPQELLEWE LVVLGKLKWN LAAVTPHDFI EHILRKLPQQ REKLSLIRKH AQTFIALCAT DFKFAMYPPS MIATGSVGAA ICGLQQDEEV SSLTCDALTE LLAKITNTDV DCLKACQEQI EAVLLNSLQQ YRQDQRDGSK SEDELDQAST PTDVRDIDL; Positive Samples: HeLa, NIH/3T3, Mouse lung, Mouse spleen, Rat lung; Cellular Location: Cytoplasm, Membrane, Nucleus
Target Information
Cyclin D2 is a G1 cyclin required for G1 phase progression and is a strong candidate for a proto-oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ccnd2; KIAK0002; MGC102758; unnamed protein product; vin-1 proto-oncogene
Gene Aliases: 2600016F06Rik; cD2; KIAK0002; MPPH3; Vin-1; Vin1
UniProt ID: (Human) P30279, (Mouse) P30280, (Rat) Q04827
Entrez Gene ID: (Human) 894, (Mouse) 12444, (Rat) 64033
G1/S transition of mitotic cell cycle
positive regulation of cell proliferation
negative regulation of apoptotic process
positive regulation of G1/S transition of mitotic cell cycle
positive regulation of mitotic cell cycle phase transition
regulation of G1/S transition of mitotic cell cycle
long-term memory
adult locomotory behavior
positive regulation of epithelial cell proliferation
regulation of cell cycle
positive regulation of cardiac muscle cell proliferation
cellular response to X-ray
negative regulation of cardiac muscle cell differentiation
ovarian follicle development
liver development
spermatogenesis
response to follicle-stimulating hormone
response to estradiol
cellular response to insulin stimulus
response to testosterone
response to genistein
response to peptide hormone
response to estrogen
response to human chorionic gonadotropin
response to ethanol
regulation of neuron differentiation
response to cAMP
cellular response to follicle-stimulating hormone stimulus
cellular response to growth hormone stimulus
cellular response to testosterone stimulus
cellular response to dexamethasone stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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