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Invitrogen
PLK1 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96158
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.91 mg/mL
Amount
91 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, HepG2, SKOV3, SGC-7901, Mouse testis; Cellular Location: Chromosome, Cytoplasm, Midbody, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle
Target Information
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; unnamed protein product
Gene Aliases: PLK; PLK1; STPK13
UniProt ID: (Mouse) Q07832, (Rat) Q62673
Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
microtubule binding
anaphase-promoting complex binding
protein kinase binding
identical protein binding
protein serine kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
nuclear division
mitotic cytokinesis
microtubule bundle formation
double-strand break repair
protein phosphorylation
mitotic spindle organization
sister chromatid cohesion
mitotic chromosome condensation
mitotic nuclear envelope disassembly
metaphase/anaphase transition of mitotic cell cycle
mitotic spindle assembly checkpoint
mitotic G2 DNA damage checkpoint
centrosome cycle
regulation of mitotic cell cycle
regulation of cell cycle process
regulation of mitotic metaphase/anaphase transition
astral microtubule organization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of cytokinesis
negative regulation of apoptotic process
establishment of protein localization
positive regulation of mitotic metaphase/anaphase transition
Golgi inheritance
regulation of cell cycle
protein localization to chromatin
double-strand break repair via alternative nonhomologous end joining
positive regulation of protein localization to nucleus
regulation of mitotic spindle assembly
regulation of mitotic cell cycle phase transition
mitotic cleavage furrow formation
regulation of protein localization to cell cortex
positive regulation of mitotic nuclear envelope disassembly
regulation of anaphase-promoting complex-dependent catabolic process
negative regulation of double-strand break repair via homologous recombination
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
cytokinesis
cell cycle
mitotic nuclear division
positive regulation of peptidyl-threonine phosphorylation
phosphorylation
female meiosis chromosome segregation
protein ubiquitination
peptidyl-serine phosphorylation
G2 DNA damage checkpoint
protein destabilization
polar body extrusion after meiotic divisions
regulation of protein binding
homologous chromosome segregation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of proteolysis
centrosome organization
cell division
positive regulation of ubiquitin-protein transferase activity
synaptonemal complex disassembly
positive regulation of ubiquitin protein ligase activity
response to antibiotic
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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