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Invitrogen
UPF2 Polyclonal Antibody
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FIGURE: 1 / 1
UPF2 Antibody (PA5-96574) in WB
Western blot analysis of UPF2 in various lysates. Samples were incubated with UPF2 Polyclonal antibody (Product # PA5-96574) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-96574
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_5421661)
Conjugate
Form
Liquid
Concentration
1.17 mg/mL
Amount
117 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: GPPLGGGEGE AESADTMPFV MLTRKGNKQQ FKILNVPMSS QLAANHWNQQ QAEQEERMRM KKLTLDINER QEQEDYQEML QSLAQRPAPA NTNRERRPRY QHPKGAPNAD LIFKTGGRRR; Positive Samples: HT-29, Jurkat, HepG2, Mouse brain; Cellular Location: Cytoplasm, perinuclear region
Target Information
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FRS2/UPF2/LEMD3 fusion; hUpf2; involved in NMD pathway; LEMD3/UPF2 fusion; nonsense mRNA reducing factor 2; Regulator of nonsense transcripts 2; smg-3 homolog, nonsense mediated mRNA decay factor; unnamed protein product; Up-frameshift suppressor 2 homolog; Upf2; UPF2 regulator of nonsense transcripts homolog; yeast Upf2p homolog
Gene Aliases: HUPF2; KIAA1408; RENT2; smg-3; UPF2
UniProt ID: (Human) Q9HAU5, (Mouse) A2AT37
Entrez Gene ID: (Human) 26019, (Mouse) 326622
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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