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Invitrogen
Phospho-MAPK15 (Thr175, Tyr177) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-MAPK15 (Thr175, Tyr177) Antibody (PA5-99135) in WB
Western blot analysis of Phospho-MAPK15 (Thr175, Tyr177) in Jurkat cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with Phospho-MAPK15 (Thr175, Tyr177) polyclonal antibody (Product # PA5-99135).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99135
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAPK15(Accession Q8TD08), corresponding to amino acid residues around phosphorylated Thr175.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of ERK8 only when phosphorylated at Threonine 175 and Tyrosine 177.
Target Information
The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.11.24; ERK-7; ERK-8; ERK8 delta; protein kinase; MAP kinase family protein; extracellular regulated kinase 8 delta; extracellular signal-regulated kinase 7; extracellular signal-regulated kinase 8; MAP kinase 15; MAPK 15
Gene Aliases: ERK-8; ERK7; ERK8
UniProt ID: (Human) Q8TD08, (Mouse) Q80Y86
Entrez Gene ID: (Human) 225689, (Mouse) 332110
MAPK cascade
cellular response to DNA damage stimulus
endoplasmic reticulum organization
positive regulation of cell proliferation
regulation of autophagy
negative regulation of cell migration
positive regulation of telomere maintenance
cellular response to stress
intracellular signal transduction
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
COPII vesicle coating
dopamine uptake
regulation of cilium assembly
protein localization to ciliary transition zone
positive regulation of metaphase/anaphase transition of meiosis I
positive regulation of spindle assembly
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
protein phosphorylation
negative regulation of DNA replication
positive regulation of protein ubiquitination
response to estradiol
positive regulation of protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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