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Invitrogen
Cytokeratin 14/16 Polyclonal Antibody
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FIGURE: 1 / 2
Cytokeratin 14/16 Antibody (PA5-99310) in IHC (P)
Immunohistochemistry analysis of Cytokeratin 14/16 in human colon cancer tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Cytokeratin 14/16 polyclonal antibody (Product # PA5-99310) using a dilution of 1:100 (1.5 hours at 22°C) followed by HRP conjugated goat anti-rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99310
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KRT14(Accession P02533), corresponding to amino acid residues M1-A36.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Cytokeratin 14/16.
Target Information
In Bowen's disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of Cytokeratin 14. Mutations in the gene encoding human Cytokeratin 14 lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK 14; CK 16; CK-14; cytokeratin 14; cytokeratin 16; cytokeratin-14; cytokeratin-16; Cytokeratin14; Cytokeratin16; epidermal keratin type I; focal non-epidermolytic palmoplantar keratoderma; intermediate filament protein; intermediate filament protein K16; K14; keratin 14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 14-like; keratin 16 homolog; keratin 16, type I; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 16; keratin intermediate filament 16a; keratin intermediate filament 16b; type I keratin Ka14; type I keratin KA16; unnamed protein product
Gene Aliases: CK-14; CK-16; CK14; CK16; EBS1; EBS1A; EBS1B; EBS1C; EBS1D; EBS3; EBS4; FNEPPK; K14; K16; K1CP; Ka14; Ka16; Krt-1.14; Krt1-14; Krt1-16; Krt14; Krt14l; KRT16A; NEPPK; NFJ; PC1
UniProt ID: (Human) P02533, (Human) P08779, (Mouse) Q61781, (Rat) Q6IFV1, (Mouse) Q9Z2K1
Entrez Gene ID: (Human) 3861, (Human) 3868, (Mouse) 16664, (Rat) 287701, (Mouse) 16666, (Rat) 303530
morphogenesis of an epithelium
epidermis development
keratinocyte differentiation
hair cycle
intermediate filament organization
intermediate filament bundle assembly
positive regulation of keratinocyte differentiation
inflammatory response
cytoskeleton organization
negative regulation of cell migration
keratinization
innate immune response
keratinocyte migration
establishment of skin barrier
response to radiation
epithelial cell differentiation
intermediate filament cytoskeleton organization
response to zinc ion
response to ionizing radiation
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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