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Invitrogen
ZNF148 Polyclonal Antibody
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FIGURE: 1 / 3
ZNF148 Antibody (PA5-99320) in ICC/IF
Immunofluorescent analysis of ZNF148 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with ZNF148 polyclonal antibody (Product # PA5-99320) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZNF148(Accession Q9UQR1), corresponding to amino acid residues L64-V114.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ZNF148.
Target Information
ZNF148 is involved in transcriptional regulation. ZNF148 represses the transcription of a number of genes including gastrin, stromelysin and enolase. ZNF148 binds to the G-rich box in the enhancer region of these genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta enolase repressor factor 1; Beta-Enolase Repressor Factor-1; binding factor of a type-I collagen promoter; CACCC box-binding protein; CLL-associated antigen KW-10; G-rich box-binding protein; transcription factor; transcription factor BFCOL1; transcription factor ZBP-89; unnamed protein product; zinc finger DNA-binding protein 89
Gene Aliases: 2210405J08Rik; BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52; ZBP-89; ZFP148; Znf148
UniProt ID: (Human) Q9UQR1, (Mouse) Q61624, (Rat) Q62806
Entrez Gene ID: (Human) 7707, (Mouse) 22661, (Rat) 58820
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter
cellular defense response
negative regulation of gene expression
substantia nigra development
negative regulation of transcription, DNA-templated
gamete generation
positive regulation of transcription from RNA polymerase II promoter
protein-containing complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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