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Invitrogen

NOTCH2 (Cleaved Asp1733) Polyclonal Antibody

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Product Details

PA5-99443

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NOTCH2(Accession Q04721), corresponding to amino acid residues L1717-S1741.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818376

Product Specific Information

Antibody detects endogenous levels of fragment of activated Notch2 resulting from cleavage adjacent to Asp1733.

Target Information

NOTCH2 (Cleaved Asp1733) refers to the activated intracellular domain of the Notch2 receptor generated following gamma-secretase-mediated proteolytic cleavage at aspartic acid residue 1733. NOTCH2 is encoded by the NOTCH2 gene and functions as a single-pass transmembrane receptor in the conserved Notch signaling pathway, which regulates cell fate determination, differentiation, proliferation, and survival. Upon ligand engagement by Delta-like or Jagged family members on adjacent cells, NOTCH2 undergoes sequential proteolytic cleavages that culminate in intramembrane cleavage and release of the Notch2 intracellular domain (NICD2). The cleaved intracellular domain translocates to the nucleus, where it associates with the DNA-binding protein RBPJ and transcriptional co-activators to drive expression of target genes, including members of the HES and HEY families. Detection of NOTCH2 cleaved at Asp1733 is widely used as a marker of active Notch2 signaling in developmental biology, stem cell regulation, immunology, and cancer research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: membrane bound receptor; Motch B; notch 2; Notch gene homolog 2; Notch homolog 2; putative cell-fate determining protein; transmembrane protein; unnamed protein product

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Gene Aliases: AGS2; HJCYS; hN2; N2

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UniProt ID: (Human) Q04721, (Mouse) O35516

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Entrez Gene ID: (Human) 4853, (Rat) 29492, (Mouse) 18129

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Function(s)
transcription coactivator activity calcium ion binding protein binding enzyme binding signaling receptor activity NF-kappaB binding
Process(es)
cell fate determination heart looping marginal zone B cell differentiation atrioventricular node development pulmonary valve morphogenesis regulation of transcription, DNA-templated apoptotic process Notch signaling pathway nervous system development axon guidance positive regulation of cell proliferation organ morphogenesis negative regulation of gene expression positive regulation of keratinocyte proliferation hemopoiesis cell differentiation intracellular signal transduction wound healing regulation of apoptotic process negative regulation of apoptotic process positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of Ras protein signal transduction bone remodeling regulation of developmental process positive regulation of smooth muscle cell differentiation cardiac septum morphogenesis atrial septum morphogenesis positive regulation of ERK1 and ERK2 cascade left/right axis specification cellular response to tumor cell proximal tubule development glomerular visceral epithelial cell development glomerular capillary formation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of hemopoiesis regulation of osteoclast development negative regulation of transcription from RNA polymerase II promoter in utero embryonic development liver development placenta development morphogenesis of an epithelial sheet inflammatory response to antigenic stimulus regulation of transcription from RNA polymerase II promoter humoral immune response determination of left/right symmetry brain development embryonic limb morphogenesis positive regulation of BMP signaling pathway activation of protein kinase B activity multicellular organism growth intrahepatic bile duct development tissue regeneration defense response to bacterium myeloid dendritic cell differentiation positive regulation of apoptotic process animal organ development system development placenta blood vessel development ciliary body morphogenesis hepatocyte proliferation liver morphogenesis cholangiocyte proliferation epithelial cell development positive regulation of cell development epithelial cell differentiation involved in kidney development developmental growth nephron tubule development
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