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Invitrogen
RAB34 Polyclonal Antibody
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FIGURE: 1 / 5
RAB34 Antibody (PA5-99697) in ICC/IF
Immunocytochemistry analysis of RAB34 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAB34 polyclonal antibody (Product # PA5-99697) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99697
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RAB34(Accession Q9BZG1), corresponding to amino acid residues K144-Y194.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Rab 34.
Target Information
Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region. Plays a role in the maturation of phagosomes that engulf pathogens, such as S. aureus and M. tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: nine amino-acid residue-repeats; RAB34, member of RAS oncogene family; ras-related protein Rab-39; Ras-related protein RAB34; ras-related protein Rah; unnamed protein product
Gene Aliases: NARR; OFD20; RAB39; RAH
UniProt ID: (Human) P0DI83, (Rat) Q5U1Y1
Entrez Gene ID: (Human) 83871, (Rat) 360571
endocytosis
lysosomal transport
antigen processing and presentation
lysosome localization
Golgi to plasma membrane protein transport
macropinocytosis
positive regulation of smoothened signaling pathway
positive regulation of protein secretion
cilium morphogenesis
cytosolic ciliogenesis
cellular response to glucose stimulus
protein localization to plasma membrane
phagosome maturation
phagosome-lysosome fusion
positive regulation of defense response to bacterium
positive regulation of protein localization to phagocytic vesicle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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