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Invitrogen
C2orf33 Polyclonal Antibody
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FIGURE: 1 / 7
C2orf33 Antibody (PA5-99963) in ICC/IF
Immunofluorescent analysis of C2orf33 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with C2orf33 polyclonal antibody (Product # PA5-99963) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99963
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MFF(Accession Q9GZY8), corresponding to amino acid residues F129-S179.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MFF.
Target Information
Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles. [Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mitochondrial fission factor
Gene Aliases: 5230400G24Rik; AI314724; Mff; RGD1310230
UniProt ID: (Rat) Q4KM98, (Mouse) Q6PCP5
Entrez Gene ID: (Rat) 301563, (Mouse) 75734
mitochondrial fission
release of cytochrome c from mitochondria
protein targeting to mitochondrion
mitochondrial fusion
regulation of mitochondrion organization
peroxisome fission
mitochondrial fragmentation involved in apoptotic process
protein homooligomerization
mitochondrion morphogenesis
positive regulation of mitochondrial fission
positive regulation of release of cytochrome c from mitochondria
positive regulation of protein targeting to membrane
regulation of peroxisome organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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