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Product Details
PMC0046
Product Specifications
Species
Mouse
Expression System
E. coli
Amino acid sequence
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Molecular weight
13.5 kDa
Class
Recombinant
Type
Protein
Purity
>96% by SDS-PAGE and RP-HPLC
Activity
ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of mouse HT-2 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized mIL-4 should be reconstituted in sterile, 10 mM HAc not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. For long term storage, it is recommended to add a carrier protein, such as 0.1% HSA or BSA.
Storage: This lyophilized preparation is stable at room temperature for 3 weeks, but should be stored desiccated below -18°C. Upon reconstitution, the preparation is stable at 4°C for up to 1 week, but should be stored below -18°C for long term storage. Avoid repeated freeze/thaw cycles.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: BSF-1; Il-4; Il4
UniProt ID: (Mouse) P07750
Entrez Gene ID: (Mouse) 16189
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