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PRKCI (PKC iota) Recombinant Human Protein

Catalog number:  PV3183

Related applications:

Protein Assays and Analysis

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Description

The PKC family of serine⁄threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NP_002731
Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
Kinase Group: Ser⁄Thr Kinases: AGC Group
Shipping Condition: Dry Ice
System Compatibility: LanthaScreen® Antibody, LanthaScreen® Substrate, Z'-LYTE™, Omnia® Bulk Substrate
Target Entry: PRKCI
Target Gene: protein kinase C, iota
Expression System: Baculovirus
Protein Family: Kinases & Inhibitors
Protein Form: Full Length, Recombinant
Protein Subtype: Serine⁄Threonine Kinases
Research Category: Signal Transduction
Species: Human
Validated Application: Kinase Assay
Gene ID (Entrez): 5584
Molecular Weight: 71.5 kDa
Gene Alias: PRKCI (PKC iota)
KinaseFamily: PKC Kinase Family
Protein Tag: His Tag

Contents & storage

Storage Buffer: 20mM HEPES, pH 7.5, 150mM NaCl, 0.5mM EGTA, 0.5mM EDTA, 0.05% NP-40, 5mM DTT, 50% glycerol
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.