4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).
For use in combination with the LanthaScreen™ Tb-anti-4E-BP1 [pThr46] Antibody (PV4757) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay Entry
Biochemical terbium phospho-specific activity
Detection Method
Fluorescent
Form
Liquid
Quantity
20 nmol
Substrate
4E-BP1
Substrate Properties
Protein-Based Substrate
Substrate Type
Kinase Substrate
Target Enzyme
Serine/Threonine Kinases
Label or Dye
GFP (EmGFP)
Molecular Weight (g/mol)
45600 Da
Shipping Condition
Dry Ice
Target Entry
mTOR, FRAP1
Validated Kinases
FRAP1 (mTOR), FRAP1 (mTOR)
Contents & Storage
GFP-4E-BP1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 2mM DTT. Store at -80°C.