Kinase Tracer 314, 25 µM

Catalog number:  PV6087

Related applications:

Pharma & Biopharma

Back to top

Description

In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or “tracer" such as Kinase Tracer 314 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Learn More and See Which Kinases Have Been Validated Using This Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Kinase Binding Assay
Assay Entry: Biochemical binding/kinetic
Excitation/Emission: 340⁄615, 665
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Label or Dye: Alexa Fluor™ 647
Readout: Kinetic
Shipping Condition: Dry Ice
Target Entry: PIK3CG, PIK3CD/PIK3R1, mTOR, FRAP1
Target Enzyme: Kinases (Multiple)
Technique: TR-FRET
Validated Kinases: PIK3CD, PIK3CG, FRAP1 (mTOR)

Contents & storage

Store at -20°C.