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Thermo Scientific™

TGFBR2 Recombinant Human Protein

Catalog number:  PV6122

Related applications:

Protein Assays and Analysis

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Description

This recombinant fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
Expressed in insect cells
GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format, with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer’ to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

TGFBR2 for the LanthaScreen Kinase Binding Assay
Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).

This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
 Tracer 178 (Cat No. PV5593)
 Eu-labeled GST Antibody (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Accession Number: NP_003233
Assay Entry: Biochemical binding/kinetic
Kinase Group: Ser⁄Thr Kinases: TKL Group
Product Line: LanthaScreen®
Readout: Kinetic
Shipping Condition: Dry Ice
System Compatibility: LanthaScreen®
Tag Position: N-Terminal
Target Entry: TGFBR2
Expression System: Baculovirus
Form: Frozen
Protein Family: Kinases & Inhibitors
Protein Form: Truncated
Protein Subtype: Serine⁄Threonine Kinases
Research Category: Signal Transduction
Species: Human
Validated Application: Kinase Assay
Gene ID (Entrez): 7048
Molecular Weight: 70.3 kDa
Purification Method: Purified
Gene Alias: TGFBR2
KinaseFamily: STKR Kinase Family
Protein Tag: GST Tag

Contents & storage

Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.