Thermo Scientific™

GRK1 Recombinant Human Protein

Catalog number: PV6352
Thermo Scientific™

GRK1 Recombinant Human Protein

Catalog number: PV6352
Catalog Number
PV6352
Unit Size
10 µg
Price (USD)
451.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 µg
Price (USD)
1,546.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 mg
Price (USD)
6,920.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV635210 µg
451.00
Your Price:
***
PV6353100 µg
1,546.00
Your Price:
***
PV63541 mg
6,920.00
Your Price:
***
Product Overview
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GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated. Full-length recombinant human GRK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry
Biochemical binding/kinetic
Kinase Group
Serine/Threonine Kinase
Target Entry
GRK1
Expression System
Baculovirus
Protein Family
Growth Factors & Receptors, Kinases & Inhibitors
Protein Form
Recombinant
Species
Human
Validated Application
Kinase Assay
Molecular Weight
89.8 kDa
Quantity
10 µg
Gene Alias
GPRK1, RHOK, RK
Recombinant
Yes
Protein Tag
GST Tag

Contents & Storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.

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