SIK1 Recombinant Human Protein

Catalog number:  PV6445

Related applications:

Pharma & Biopharma

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Description

SIK1 is a protein kinase that is involved in regulating AMPK-related kinases. This recombinant human SIK1 (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance

SIK1 may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells.

Learn more about our kinase proteins >

Related Links:
LanthaScreen® Kinase Activity Assay
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NP_173354
Assay Entry: Biochemical binding/kinetic
Kinase Group: Serine/Threonine Kinase
Target Entry: SIK1
Expression System: Baculovirus
Protein Form: Recombinant, Active, Catalytic
Research Category: Drug Development, Signal Transduction
Species: Human
Validated Application: Kinase Assay
Gene ID (Entrez): 150094
Molecular Weight: 61 kDa
Gene Alias: SNF1LK, MSK, SIK1
Protein Length: aa 1-303
Protein Tag: GST Tag

Contents & storage

Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol

Store at -68 to -85°C. After first use, aliquot and store to avoid multiple freeze-thaws.