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Invitrogen
F(ab')2-Goat anti-Human IgG (H+L) Secondary Antibody, Qdot™ 655
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FIGURE: 1 / 1
Human IgG (H+L) Secondary Antibody (Q-11221MP) in mIHC
A mouse intestinal section visualized using fluorescent Qdot® nanocrystal conjugates. Actin was labeled with a mouse anti-actin monoclonal antibody and visualized using red-fluorescent Qdot® 655 goat F (ab')2 anti-mouse IgG (Product # Q11022MP, Product # Q-11221MP). Laminin was labeled with a rabbit anti-laminin polyclonal antibody and visualized using green-fluorescent Qdot® 525 goat F (ab')View More
Product Details
Q-11221MP
Applications
Tested Dilution
Western Blot (WB)
1:50
Immunohistochemistry (IHC)
1:50
Immunocytochemistry (ICC/IF)
1:50
Flow Cytometry (Flow)
1:50
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
300/654 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
0.05M borate, pH 8.3, with 1M betaine
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
F(ab')2 Fragment
Product Specific Information
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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