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Invitrogen
Goat anti-Chicken IgY (H+L) Secondary Antibody, Qdot™ 655
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FIGURE: 1 / 3
Chicken IgY (H+L) Secondary Antibody (Q14421MP) in WB
Multiplexed fluorescent western blot was performed using Goat anti-Chicken IgY (H+L) Secondary Antibody, Qdot™ 655 (Product # Q14421MP). Whole cell extracts of MCF 10A (Lane 1, 2, 3), SHSY-5Y (Lane 4, 5, 6), HaCaT (Lane 7), HeLa (Lane 8), MCF7 (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03221BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Prod... View More
Product Details
Q14421MP
Applications
Tested Dilution
Western Blot (WB)
1:50-1:500
Immunohistochemistry (IHC)
1:50
Immunocytochemistry (ICC/IF)
1:50
Flow Cytometry (Flow)
1:50
Product Specifications
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Chicken IgY (H+L)
Conjugate
Excitation/Emission Max
300/654 nm
View spectra
Form
Liquid
Concentration
1 µM
Storage conditions
4°C, store in dark
Shipping conditions
Ambient
RRID
Target
IgY
Antibody Form
Whole Antibody
Product Specific Information
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Target Information
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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