What is the best way to remove white precipitate from my ITK Qdot nanocrystals?
Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?
The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Why do my Qdot nanocrystals appear to be blinking?
Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?
Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:
HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Why can't I freeze my Qdot nanocrystal solution?
Freezing will cause the product to aggregate. The Qdot nanocrystals cannot be dispersed into solution after aggregation.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
