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Invitrogen
Donkey anti-Mouse IgG (H+L) Secondary Antibody, Qdot™ 525
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FIGURE: 1 / 3
Mouse IgG (H+L) Secondary Antibody (Q22073) in WB
Multiplexed fluorescent western blot was performed using Donkey anti-Mouse IgG (H+L) Secondary Antibody, Qdot™ 525 (Product # Q22073). Whole cell extracts of A-431 (Lane 1, 2, 3), HaCaT (Lane 4, 5, 6), SH-SY5Y (Lane 7), HeLa (Lane 8) and MCF7 (Lane 9) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03221BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product... View More
Product Details
Q22073
Applications
Tested Dilution
Western Blot (WB)
1:50-1:500
Immunohistochemistry (IHC)
1:50
Immunocytochemistry (ICC/IF)
1:50
Flow Cytometry (Flow)
1:50
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
300/525 nm
View spectra
Form
Liquid
Concentration
1 µM
Purification
purified
Storage buffer
0.05M borate, pH 8.3, with 1M betaine
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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