Qubit™ RNA IQ Assay Kit, 275 assays

Qubit™ RNA IQ Assay Kit, 275 assays - FAQs

View additional product information for Qubit™ RNA IQ Assay Kits - FAQs (Q33222, Q33221)

5 product FAQs found

Can the Qubit RNA IQ Assay Kits (Cat. No. Q33221, Q33222) be run on a plate reader for high throughput screening of RNA integrity?

Qubit RNA IQ Assay Kits are not compatible with plate readers. The IQ value calculations are performed by the Qubit instrument, which involves proprietary algorithms that cannot be replicated on other devices. For accurate results, use the Qubit instrument as recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using the Qubit RNA IQ Assay Kit (Cat. No. Q33221, Q33222). How do I convert the RNA IQ score to the RIN score?

There is no simple way to convert the RNA IQ score to RIN, because they measure different things. The application note, Qubit RNA IQ Assay: a fast and easy fluorometric RNA quality assessment, shows a detailed comparison and explanation of the two measurements. Figures 2 and 4 are most relevant.

Does the Qubit RNA IQ Assay detect free nucleotides/bases?

No. The presence of an excess amount of free nucleotides/bases may affect the assay results in the same manner as other contaminants, but the reagents in this kit do not give a fluorescence response to nucleotides or bases.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer a Qubit assay to detect purity of the RNA or DNA samples (level of protein contamination)?

At present, we do not have a fluorescence-based assay that tests for sample purity (protein contamination). We do offer various instruments that can obtain absorbance readings (absorbance at 260/280), such as our NanoDrop, BioMate and GENESYS spectrophotometers.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does the Qubit RNA IQ Assay detect the purity of the RNA sample, similar to using the 260/280 absorbance method?

No. The Qubit RNA IQ Assay does not assay the purity of the RNA sample, only the extent of degradation of the RNA (small versus large strands).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.