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Image-iT™ Fixation/Permeabilization Kit - FAQs

Image-iT™ Fixation/Permeabilization Kit - FAQs

View additional product information for Image-iT™ Fixation/Permeabilization Kit - FAQs (R37602)

3 product FAQs found

The Image-iT Fixation/Permeabilization Kit includes Triton X-100 as the permeabilizing reagent. May I substitute other reagents for permeabilizing?

Yes. Aldehyde-based fixation is compatible with other permeabilizing reagents, such as saponin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the Image-iT Fixative Solution alcohol-free?

Yes, it is alcohol-free.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I add the Image-iT Fixative Solution in with the media?

We do not recommend doing this. The Image-iT Fixative Solution is 4% formaldehdye in PBS, so mixing it in directly with media would dilute the final concentration of fixative and this may result in less than optimal fixation. If you need to add fixative directly to the medium, consider using a fixative at a higher concentration such as formaldehyde at 16% (Cat. No. 28906) or at 37% (Cat. No. 9311).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.