Multipotent stem cells from trabecular meshwork become phagocytic TM cells.
AuthorsDu Y, Roh DS, Mann MM, Funderburgh ML, Funderburgh JL, Schuman JS,
JournalInvest Ophthalmol Vis Sci
PubMed ID22297497
'To isolate and characterize stem cells from human trabecular meshwork (TM) and to investigate the potential of these stem cells to differentiate into TM cells. Human trabecular meshwork stem cells (TMSCs) were isolated as side population cells by fluorescence-activated cell sorting or isolated by clonal cultures. Passaged TMSCs were compared ... More
Transitions of protein traffic from cardiac ER to junctional SR.
AuthorsSleiman NH, McFarland TP, Jones LR, Cala SE,
Journal
PubMed ID25640161
'The junctional sarcoplasmic reticulum (jSR) is an important and unique ER subdomain in the adult myocyte that concentrates resident proteins to regulate Ca(2+) release. To investigate cellular mechanisms for sorting and trafficking proteins to jSR, we overexpressed canine forms of junctin (JCT) or triadin (TRD) in adult rat cardiomyocytes. Protein ... More
DNA polymerase ß-dependent cell survival independent of XRCC1 expression.
AuthorsHorton JK, Gassman NR, Dunigan BD, Stefanick DF, Wilson SH,
Journal
PubMed ID25541391
'Base excision repair (BER) is a primary mechanism for repair of base lesions in DNA such as those formed by exposure to the DNA methylating agent methyl methanesulfonate (MMS). Both DNA polymerase ß (pol ß)- and XRCC1-deficient mouse fibroblasts are hypersensitive to MMS. This is linked to a repair deficiency ... More
Co-storage and secretion of growth hormone and secretoneurin in retinal ganglion cells.
AuthorsMartinez-Moreno CG, Trudeau VL, Harvey S,
Journal
PubMed ID25435278
'It is well established that growth hormone (GH) and granins are co-stored and co-secreted from pituitary somatotrophs. In this work we demonstrate for the first time that GH- and secretoneurin (SN) immunoreactivity (the secretogranin II (SgII) fragment) are similarly present in retinal ganglion cells (RGCs), which is an extrapituitary site ... More
Molecular mechanism of sphingosine-1-phosphate action in Duchenne muscular dystrophy.
AuthorsNguyen-Tran DH, Hait NC, Sperber H, Qi J, Fischer K, Ieronimakis N, Pantoja M, Hays A, Allegood J, Reyes M, Spiegel S, Ruohola-Baker H,
Journal
PubMed ID24077965
'Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease. Studies in Drosophila showed that genetic increase of the levels of the bioactive sphingolipid sphingosine-1-phosphate (S1P) or delivery of 2-acetyl-5-tetrahydroxybutyl imidazole (THI), an S1P lyase inhibitor, suppresses dystrophic muscle degeneration. In the dystrophic mouse (mdx), upregulation of S1P by THI increases ... More
Cell fate inclination within 2-cell and 4-cell mouse embryos revealed by single-cell RNA sequencing.
AuthorsBiase FH, Cao X, Zhong S,
Journal
PubMed ID25096407
'It remains an open question when and how the first cell fate decision is made in mammals. Using deep single-cell RNA-seq of matched sister blastomeres, we report highly reproducible inter-blastomere differences among 10 2-cell and five 4-cell mouse embryos. Inter-blastomere gene expression differences dominated between-embryo differences and noise, and were ... More
6-Phosphogluconate dehydrogenase regulates tumor cell migration in vitro by regulating receptor tyrosine kinase c-Met.
AuthorsChan B, VanderLaan PA, Sukhatme VP,
Journal
PubMed ID23973484
'6-Phosphogluconate dehydrogenase (6PGD) is the third enzyme in the oxidative pentose phosphate pathway (PPP). Recently, we reported that knockdown of 6PGD inhibited lung tumor growth in vitro and in a xenograft model in mice. In this study, we continued to examine the functional role of 6PGD in cancer. We show ... More
In vitro screening test using Leishmania promastigotes stably expressing mCherry protein.
AuthorsVacchina P, Morales MA,
Journal
PubMed ID24395225
Transgenic Leishmania major and Leishmania donovani axenic promastigotes constitutively expressing mCherry were used for in vitro antileishmanial drug screening. This method requires minimal sample manipulation and can be easily adapted to automatic drug tests, allowing primary high-throughput screenings without the need for expensive and sophisticated instruments. ... More
A plasmid-based reporter system for live cell imaging of dengue virus infected cells.
AuthorsMedin CL, Valois S, Patkar CG, Rothman AL,
Journal
PubMed ID25445884
Cell culture models are used widely to study the effects of dengue virus (DENV) on host cell function. Current methods of identification of cells infected with an unmodified DENV requires fixation and permeablization of cells to allow DENV-specific antibody staining. This method does not permit imaging of viable cells over ... More
Notch3-Jagged signaling controls the pool of undifferentiated airway progenitors.
AuthorsMori M, Mahoney JE, Stupnikov MR, Paez-Cortez JR, Szymaniak AD, Varelas X, Herrick DB, Schwob J, Zhang H, Cardoso WV,
Journal
PubMed ID25564622
Basal cells are multipotent airway progenitors that generate distinct epithelial cell phenotypes crucial for homeostasis and repair of the conducting airways. Little is known about how these progenitor cells expand and transition to differentiation to form the pseudostratified airway epithelium in the developing and adult lung. Here, we show by ... More
Extracellular DNA Impedes the Transport of Vancomycin in Staphylococcus epidermidis Biofilms Preexposed to Subinhibitory Concentrations of Vancomycin.
Staphylococcus epidermidis biofilm formation is responsible for the persistence of orthopedic implant infections. Previous studies have shown that exposure of S. epidermidis biofilms to sub-MICs of antibiotics induced an increased level of biofilm persistence. BODIPY FL-vancomycin (a fluorescent vancomycin conjugate) and confocal microscopy were used to show that the penetration ... More
Human fibroblast-derived extracellular matrix constructs for bone tissue engineering applications.
AuthorsTour G, Wendel M, Tcacencu I,
JournalJ Biomed Mater Res A
PubMed ID23471711
We exploited the biomimetic approach to generate constructs composed of synthetic biphasic calcium phosphate ceramic and extracellular matrix (SBC-ECM) derived from adult human dermal fibroblasts in complete xeno-free culture conditions. The construct morphology and composition were assessed by scanning electron microscopy, histology, immunohistochemistry, Western blot, glycosaminoglycan, and hydroxyproline assays. Residual ... More
An iron-regulated and glycosylation-dependent proteasomal degradation pathway for the plasma membrane metal transporter ZIP14.
AuthorsZhao N, Zhang AS, Worthen C, Knutson MD, Enns CA,
Journal
PubMed ID24927598
Protein degradation is instrumental in regulating cellular function. Plasma membrane proteins targeted for degradation are internalized and sorted to multivesicular bodies, which fuse with lysosomes, where they are degraded. ZIP14 is a newly identified iron transporter with multitransmembrane domains. In an attempt to dissect the molecular mechanisms by which iron ... More
Apoptosis-inducing factor (AIF) is targeted in IFN-a2a-induced Bid-mediated apoptosis through Bak activation in ovarian cancer cells.
AuthorsMiyake K, Bekisz J, Zhao T, Clark CR, Zoon KC,
JournalBiochim Biophys Acta
PubMed ID22683989
Previously we have shown that interferon (IFN)-a induced apoptosis is predominantly mediated by the upregulation of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) via the caspase-8 pathway. It was also shown that recruitment of mitochondria in IFN-a induced apoptosis involves the cleavage of BH3 interacting domain death agonist (Bid) to ... More
Antigen presentation by lung epithelial cells directs CD4+ TRM cell function and regulates barrier immunity.
Authors
JournalNat Commun
PubMed ID34611166
P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.
Authors
JournalMol Cell
PubMed ID30824372
Reprogramming tumor stroma using an endogenous lipid lipoxin A4 to treat pancreatic cancer.
AuthorsSchnittert J, Heinrich MA, Kuninty PR, Storm G, Prakash J
JournalCancer Lett
PubMed ID29408203
'Pancreatic stellate cells (PSCs) are the precursors of cancer-associated fibroblasts (CAFs), which potentiate pancreatic tumor growth and progression. In this study, we investigated whether Lipoxin A4 (LXA4), an endogenous bioactive lipid, can inhibit the differentiation of human PSCs (hPSCs) into CAF-like myofibroblasts and thereby hPSC-induced pro-tumorigenic effects. LXA4 significantly inhibited ... More
The SINEB1 element in the long non-coding RNA Malat1 is necessary for TDP-43 proteostasis.
Authors
JournalNucleic Acids Res
PubMed ID31863590
Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis.
Authors
JournalImmunity
PubMed ID32428502
Synergistic Adhesiveness of Fibronectin with PHSRN Peptide in Gelatin Mixture Promotes the Therapeutic Potential of Human ES-Derived MSCs.
AuthorsKim HS, Choi SH, Kang ML, Lee KW, Kim KN, Sung HJ
JournalCell Mol Bioeng
PubMed ID32030109
'Mesenchymal stem cells (MSCs) are promising candidates for cell therapy owing to their therapeutic effect in various diseases. In general, MSCs grow efficiently in serum-containing culture media, indicating an essential role of adhesion in their mesenchymal lineage-specific propagation. Nevertheless, the use of non-human supplements in culture (xeno-free issue) in addition ... More
Transposon silencing in the
AuthorsDurdevic Z, Pillai RS, Ephrussi A
JournalLife Sci Alliance
PubMed ID30456388
'The Piwi-interacting RNA pathway functions in transposon control in the germline of metazoans. The conserved RNA helicase Vasa is an essential Piwi-interacting RNA pathway component, but has additional important developmental functions. Here, we address the importance of Vasa-dependent transposon control in the' ... More
Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms.
Authors
JournalNature
PubMed ID33239784
Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit.
Authors
JournalMol Cell
PubMed ID33357414
Morphological screening of mesenchymal mammary tumor organoids to identify drugs that reverse epithelial-mesenchymal transition.
Authors
JournalNat Commun
PubMed ID34253738
Expanding the editable genome and CRISPR-Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking.
Authors
JournalNucleic Acids Res
PubMed ID31799604
Advanced maturation of human cardiac tissue grown from pluripotent stem cells.
Authors
JournalNature
PubMed ID29618819
A Rapid Induction Mechanism for Lin28a in Trophic Responses.
Authors
JournalMol Cell
PubMed ID28132840
Super-multiplex vibrational imaging.
AuthorsWei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W
JournalNature
PubMed ID28424513
The ability to visualize directly a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes. Even though existing methods have successfully been used to explore structure-function relationships in nervous systems, to profile RNA in situ, to reveal the heterogeneity of tumour microenvironments ... More
Validation of Current Good Manufacturing Practice Compliant Human Pluripotent Stem Cell-Derived Hepatocytes for Cell-Based Therapy.
AuthorsBlackford SJI, Ng SS, Segal JM, King AJF, Austin AL, Kent D, Moore J, Sheldon M, Ilic D, Dhawan A, Mitry RR, Rashid ST
JournalStem Cells Transl Med
PubMed ID30456803
Recent advancements in the production of hepatocytes from human pluripotent stem cells (hPSC-Heps) afford tremendous possibilities for treatment of patients with liver disease. Validated current good manufacturing practice (cGMP) lines are an essential prerequisite for such applications but have only recently been established. Whether such cGMP lines are capable of ... More