ReadyProbes™ Cell Viability Imaging Kit, Blue/Green - FAQs

ReadyProbes™ Cell Viability Imaging Kit, Blue/Green - FAQs

View additional product information for ReadyProbes™ Cell Viability Imaging Kit, Blue/Green - FAQs (R37609)

3 product FAQs found

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fluorescent viability assays can I use on the Countess II FL automated cell counter?

We have validated the following kits for use on the Countess II FL Automated Cell Counter:

LIVE/DEAD Viabilty/Cytoxicity Kit (Cat. No. L3224) containing calcein AM and ethidium homodimer-1
ReadyProbes Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610) containing NucBlue Live/NucGreen Dead and NucBlue Live/propidium iodide
See this Application Note for details - https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between NucBlue Live reagents stain and NucGreen Dead reagents stain?

The NucBlue Live reagent stains the nuclei of all cells, while NucGreen Dead reagent stains only the nuclei of dead cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.