FreeStyle™ CHO-S Cells - FAQs

FreeStyle™ CHO-S Cells - FAQs

View additional product information for FreeStyle™ CHO-S Cells - FAQs (R80007)

2 product FAQs found

How do FreeStyle 293-F cells compare to FreeStyle CHO-S cells in terms of antibody and protein yield?

When comparing FreeStyle 293-F cells with FreeStyle CHO-S cells for average range of antibody and protein yield, it is definitely expected that antibody yield will be higher in FreeStyle 293-F cells than in FreeStyle CHO-S cells, when generated by transient transfection. On the other hand, antibody yields in stable CHO cells can be much higher. For non-antibody proteins, it is impossible to predict which of the two cell lines may provide higher yields.

How long can I keep culturing FreeStyle cells after transfection?

We typically keep the culture going for 7-10 days posttransfection. After that, protein expression may drop.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.