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Invitrogen
TNF alpha Monoclonal Antibody (MP6-XT22), Pacific Blue™
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FIGURE: 1 / 2
TNF alpha Antibody (RM90128) in Flow
C57BL/6 splenocytes were left unstimulated or stimulated for 5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. Cells were then surface-stained with anti-mouse CD4 FITC (Product # MCD0401) and subsequently fixed and permeabilized using the FIX & PERM® Cell Permeabilization Kit (Product # GAS-003) and stained intracellularly with anti-mouse interferon gamma (IFN-gamma) PE-Cy®7 conjugate (Product # A18713) and anti-mouse tumor necrosis factor alpha (TNF-a) Pacific Blue™ conjugate (Product # RM90128). S... View More
Product Details
RM90128
Product Specifications
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MP6-XT22
Immunogen
Mouse TNF Alpha.
Conjugate
Excitation/Emission Max
404/455 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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