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Invitrogen
Human MED4 (aa 6-120) Control Fragment Recombinant Protein
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Product Details
RP-89352
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDSDIQQLQKQLKEAEQI
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-82240. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (Sato et al., 2004).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; FLJ10956; Mediator complex subunit 4; Mediator of RNA polymerase II transcription subunit 4; mediator, 34-kD subunit, homolog; RP11-90M2.2; TRAP/SMCC/PC2 subunit p36; TRAP/SMCC/PC2 subunit p36 subunit; vitamin D receptor-interacting protein, 36-kD; Vitamin D3 receptor-interacting protein complex 36 kDa component
Gene Aliases: ARC36; DRIP36; HSPC126; MED4; TRAP36; VDRIP
UniProt ID: (Human) Q9NPJ6
Entrez Gene ID: (Human) 29079
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