Search
Invitrogen
Human Aggrecan (Interglobular Domain) Recombinant Protein
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
Human Aggrecan (Interglobular Domain) Protein (RP-77527)
Hydrolysis of recombinant aggrecan interglobular domain with isolated ADAMTS4 Recombinant aggrecan-IGD1 (Product # RP-77527) at a concentration of 10 µM in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2 was incubated without and with 0.1 µM truncated ADAMTS4 (Product # RP-77540) for various time intervals. Aliquots with 1.5 µg aggrecan-IGD1 were then analyzed by SDS-PAGE. Lane 1: Aggrecan-IGD1 incubated without ADAMTS4, Lanes 2-6: Digestion mixtures of aggrecan-IGD1 with ADAMTS4 after 0, 0.5, 1, 2 and 5 hours incubation.
Product Details
RP-77527
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
RP-77527 is a purified recombinant Aggrecan Interglobular Domain protein. The recombinant protein contains cleavage sites for aggrecanases (E373-A374 in aggrecan) and matrix metalloproteinases (N341-F342 in aggrecan). RP-77527 may be used as a control with corresponding anti-Aggrecan Interglobular Domain antibodies.
Target Information
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support