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Invitrogen
Mouse Kallikrein 1 His-tag Recombinant Protein
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Product Details
RP-88241
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse KLK1, amino acids Met1-Asp261 (Accession # P15947) with a C-terminal His-tag
Tag
His-tag
Molecular weight
36 kDa
Class
Recombinant
Type
Protein
Purity
>90% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >6, 000 pmol/min/ µg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Amount
50 µg
Purification
purified
Storage buffer
25mM tris, pH 7.4, with 5mM CaCl2, 0.01% mannitol, 0.15M NaCl, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (200 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glandular kallikrein K1; kallikrein renal/pancreas/salivary; tissue kallikrein-6; true tissue kallikrein; unnamed protein product
Gene Aliases: 0610007D04Rik; Kal; KAL-B; Klk1b6; Klk6; mGk-6
UniProt ID: (Mouse) P15947
Entrez Gene ID: (Mouse) 16612
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