SYPRO™ Tangerine Protein Gel Stain (5000X Concentrate in DMSO), 500 μL - Citations
SYPRO™ Tangerine Protein Gel Stain (5000X Concentrate in DMSO), 500 μL - Citations
View additional product information for SYPRO™ Protein Gel Stains - Citations (S12000, S12001, S6650, S6651, S12010, S12000X3, S6654, S6653, S21900)
Showing 12 product Citations
Citations & References
Abstract
Translational regulation of prostaglandin endoperoxide H synthase-1 mRNA in megakaryocytic MEG-01 cells. Specific protein binding to a conserved 20-nucleotide CIS element in the 3'-untranslated region.
AuthorsDuquette M, Laneuville O
JournalJ Biol Chem
PubMed ID12237309
Prostaglandin endoperoxide H synthase-1 (PGHS-1) is an abundant enzyme in platelets, where it plays a key role in the cascade of prostanoid formation. In platelets, the primary site of PGHS-1 synthesis is in precursor megakaryocytic cells. We have previously shown that in megakaryocytic MEG-01 cells, TPA induces an increase of ... More
Social exploitation of vitellogenin.
AuthorsAmdam GV, Norberg K, Hagen A, Omholt SW
JournalProc Natl Acad Sci U S A
PubMed ID12566563
'Vitellogenin is a female-specific glucolipoprotein yolk precursor produced by all oviparous animals. Vitellogenin expression is under hormonal control, and the protein is generally synthesized directly before yolk deposition. In the honeybee (Apis mellifera), vitellogenin is not only synthesized by the reproductive queen, but also by the functionally sterile workers. In ... More
Virulence-associated trimeric autotransporters of Haemophilus parasuis are antigenic proteins expressed in vivo.
AuthorsOlvera A, Pina S, Pérez-Simó M, Oliveira S, Bensaid A,
JournalVet Res
PubMed ID19995512
Glässer's disease is a re-emerging swine disease characterized by a severe septicaemia. Vaccination has been widely used to control the disease, although there is a lack of extended cross-protection. Trimeric autotransporters, a family of surface exposed proteins implicated in host-pathogen interactions, are good vaccine candidates. Members of this family have ... More
A method for the isolation of covalent DNA-protein crosslinks suitable for proteomics analysis.
AuthorsBarker S, Murray D, Zheng J, Li L, Weinfeld M,
JournalAnal Biochem
PubMed ID16091282
The covalent crosslinking of protein to DNA is a form of DNA damage induced by a number of commonly encountered agents, including metals, aldehydes, and radiation as well as chemotherapeutic drugs. DNA-protein crosslinks (DPCs) are potentially bulky and helix distorting and have the potential to block the progression of translocating ... More
Identification of mammalian proteins cross-linked to DNA by ionizing radiation.
AuthorsBarker S, Weinfeld M, Zheng J, Li L, Murray D,
JournalJ Biol Chem
PubMed ID16093242
Ionizing radiation (IR) is an important environmental risk factor for various cancers and also a major therapeutic agent for cancer treatment. Exposure of mammalian cells to IR induces several types of damage to DNA, including double- and single-strand breaks, base and sugar damage, as well as DNA-DNA and DNA-protein cross-links ... More
Metabolic biotinylation provides a unique platform for the purification and targeting of multiple AAV vector serotypes.
The development of rationally designed targeted gene delivery vectors is an important focus for gene therapy. While genetic modification of AAV can produce vectors with modified tropism, incorporation of targeting peptides into the structural context of the AAV virion often results in loss of function or loss of virion integrity. ... More
Histometrics: improvement of the dynamic range of fluorescently stained proteins resolved in electrophoretic gels using hyperspectral imaging.
AuthorsWoodward AM, Kaderbhai N, Kaderbhai M, Shaw A, Rowland J, Kell DB,
JournalProteomics
PubMed ID11922594
Most image-based analyses, using absorbance or fluorescence of the spatial distribution of identifiable structures in complex biological systems, use only a very small number of dimensions of possible spectral data for the generation and interpretation of the image. We here extend the concepts of hyperspectral imaging, being developed in remote ... More
Protein gel staining methods: an introduction and overview.
AuthorsSteinberg TH,
JournalMethods Enzymol
PubMed ID19892191
Laboratory scientists who encounter protein biochemistry in many of its myriad forms must often ask: is my protein pure? The most frequent response: run a denaturing SDS polyacrylamide gel. Running this gel raises another series of considerations regarding detection, quantitation, and characterization and so the next questions invariably center on ... More
Mass spectrometry compatibility of two-dimensional gel protein stains.
As proteomic technology evolves, protein staining sensitivity is constantly being improved, enabling researchers to better visualize the proteome of their system. The current challenge is to balance the limits of detection of protein visualization with those of the mass spectrometric methods. In this report, mass spectra generated from human serum ... More
A thousand points of light: the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics.
AuthorsPatton WF
JournalElectrophoresis
PubMed ID10786886
As proteomics evolves into a high-throughput technology for the study of global protein regulation, new demands are continually being placed upon protein visualization and quantitation methods. Chief among these are increased detection sensitivity, broad linear dynamic range and compatibility with modern methods of microchemical analyses. The limitations of conventional protein ... More
Making blind robots see: the synergy between fluorescent dyes and imaging devices in automated proteomics.
AuthorsPatton WF
JournalBiotechniques
PubMed ID10818702
Proteomics investigations endeavor to provide a global understanding of gene product synthesis rate, degradation rate, functional competence, posttranslational modification, subcellular distribution and physical interactions with other cell components. Protein expression encompasses an enormous dynamic range. Since rare proteins cannot be amplified by any type of PCR method, sensitive detection is ... More
Fluorescence detection of proteins in sodium dodecyl sulfate-polyacrylamide gels using environmentally benign, nonfixative, saline solution.
SYPRO Tangerine stain is an environmentally benign alternative to conventional protein stains that does not require solvents such as methanol or acetic acid for effective protein visualization. Instead, proteins can be stained in a wide range of buffers, including phosphate-buffered saline or simply 150 mM NaCl using an easy, one-step ... More