Thermo Scientific™

phiX174 RF1 DNA

Catalog number: SD0031
Thermo Scientific™

phiX174 RF1 DNA

Catalog number: SD0031
Catalog Number
SD0031
Unit Size
50 µg
Price (USD)
Price: 132.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
SD003150 µg
Price: 132.00
Your Price:
-
Product Overview
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Additional Information
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Thermo Scientific phiX174 was the first DNA virus discovered to have a single-stranded, circular genome. phiX174 DNA is 5386 nt in length. The DNA strand packaged into the virion is termed the “plus” strand. After entering the cell, the Thermo Scientific phiX174 DNA is used as a template for minus-strand synthesis, producing double-stranded DNA. The conversion of plus DNA strands to double-stranded DNA does not require any of the phage genes to function.

The double-stranded DNA can then be transcribed, resulting in synthesis of phage-encoded proteins. Synthesis of single-stranded (plus) DNA requires phage-encoded gene A protein. DNA synthesis is initiated at ori (+) and proceeds in the direction indicated. Late in infection, the single-stranded circles are encapsidated into new virions. The cycle terminates by cellular lysis, mediated by phage gene E encoded protein.

Highlights

• Purified by chromatography using proprietary patented technology
• More than 90% in the supercoiled form
• Isolated from E. coli HF4704 infected with phiX174 am3

Applications

• Preparation of DNA molecular weight standards

GenBank/EMBL Accession Numbers
V01128, J02482, M10348, M10379, M10714, M10749, M10750, M10866, M10867, M24859

phiX174 plasmid usage notes:

For DNA sequence, sequence analysis and map creation, see the REviewer free online tool
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
0.5 μg/μL
Sample Type
DNA
Final Product Type
DNA
Gel Compatibility
Agarose Gels
For Use With (Application)
Cloning/Nucleic Acid Gel Electrophoresis
Product Type
DNA Reagent
Quantity
50 μg