Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Catalog number:  T1012

 Related applications:

Protein Assays and Analysis

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The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.

• Glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

The Galacto-Star™ system includes Galacton-Star® chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.

Long-Lasting Signal
Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star® substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Assay Type: β-Gal (lacZ) Assay
Compatible Cells: Mammalian Cells
Detection Method: Chemiluminescent
For Use With (Equipment): Luminometer (Microplate)
Form: Liquid
Format: 96-well plate
High Throughput Compatibility: High Throughput-Compatible
Product Line: Galacto-Star™, NovaBright™
Product Size: 600 assays
Readout: End Point
Sample Type (Specific): Cell Cultures
Substrate: Galacton-Star®
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
Technique: Enhanced Chemiluminescence

Contents & storage

This kit contains the lysis solution, substrate, enhancer, and required buffer. Store the product at 4°C.


Manuals & protocols