Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Catalog number: T2184

Applied Biosystems™  Related applications: Protein Assays and Analysis

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This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots.

The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes.

• Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes.
• Enhancer increases signal intensity, allowing decreased signal acquisition time.

Increased Signal, Shorter Exposures
Without enhancer, the chemiluminescent signal on nitrocellulose membranes is very weak, making detection either impossible or very time consuming. With enhancer, short exposures (10 to 45 minutes) can be performed on the same material. Although PVDF membranes do not require the use of Nitro-Block-II™ enhancer, exposure times are ten-fold faster with enhancer. This permits very short exposure times on PVDF, ranging from 15 seconds to 15 minutes for western blots. Nitro-Block-II™ enhancer may be used with CSPD® substrate on nitrocellulose or PVDF membranes. Nitro-Block-II™ enhancer should only be used with CDP-Star® substrate on nitrocellulose membranes.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Format: Bottle(s)
Product Line: Nitro-Block-ll™, NovaBright™
Product Size: 20 mL
Substrate: Alkaline Phosphatase
Substrate Type: AP (Alkaline Phosphatase) Substrate
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols