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Citas y referencias (15)
Invitrogen™
Thiol and Sulfide Quantitation Kit
El kit de cuantificación de tiol y sulfuro proporciona un ensayo colorimétrico ultrasensible para cuantificar tanto los tioles proteicos comoMás información
| Número de catálogo | Cantidad |
|---|---|
| T6060 | 1 kit |
Número de catálogo T6060
Precio (MXN)
-
Cantidad:
1 kit
El kit de cuantificación de tiol y sulfuro proporciona un ensayo colorimétrico ultrasensible para cuantificar tanto los tioles proteicos como los no proteicos (también llamados sulfhidrilos o mercaptanos). En este ensayo, los tioles o los sulfuros inorgánicos reducen un derivado de la papaína inhibido por el disulfuro, liberando estequiométricamente la enzima activa. A continuación, se mide la actividad de la enzima con el sustrato cromogénico de papaína N-benzoil-L-arginina, p-nitroanilida (L-BAPNA). El paso de amplificación enzimática de este kit permite a los investigadores detectar hasta 0,2 µm de tiol, una sensibilidad que es aproximadamente 100 veces mayor que la que se consigue con el reactivo de Ellman.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Cantidad1 kit
Tipo de productoKit de cuantificación de tiol y sulfuro
Unit Size1 kit
Citations & References (15)
Citations & References
Abstract
Site-directed fluorescence studies of a prokaryotic ClC antiporter.
Journal:Biochemistry
PubMed ID:16734414
'Channels and transporters of the ClC family serve a variety of physiological functions. Understanding of their gating and transport mechanisms remains incomplete, with disagreement over the extent of protein conformational change involved. Using site-directed fluorescence labeling, we probe ClC-ec1, a prokaryotic ClC, for transport-related structural rearrangements. We specifically label cysteines
Reversible glutathionylation of complex I increases mitochondrial superoxide formation.
Journal:J Biol Chem
PubMed ID:12649289
'Increased production of reactive oxygen species (ROS) by mitochondria is involved in oxidative damage to the organelle and in committing cells to apoptosis or senescence, but the mechanisms of this increase are unknown. Here we show that ROS production by mitochondrial complex I increases in response to oxidation of the
Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.
Journal:Environ Sci Technol
PubMed ID:19031913
Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular
A sensitive assay for maleimide groups.
Journal:Bioconjug Chem
PubMed ID:7948101
A sensitive spectrophotometric assay has been developed for maleimide groups incorporated into proteins. The assay involves the reaction of maleimide with an excess of cysteine and quantitation of the remaining cysteine using an enzymatic assay that is based on the stoichiometric conversion of an inactive disulfide form of papain to
An amplified assay for thiols based on reactivation of papain.
Journal:Anal Biochem
PubMed ID:8238881
A sensitive spectrophotometric assay has been developed for thiol (sulfhydryl) groups using an inactive disulfide derivative of papain (papain-S-SCH3). The thiol-disulfide interchange reaction of a thiol with papain-S-SCH3 results in the stoichiometric formation of active papain (papain-SH). The reactivated papain catalyzes the hydrolysis of a chromogenic substrate, resulting in an