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          • Primary Antibodies ›
          • DYKDDDDK Tag Antibodies

          OriGene

          DDK Polyclonal Antibody, TrueMAB™

          View all (91) DYKDDDDK Tag antibodies

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          Cite DDK Polyclonal Antibody, TrueMAB™

          Additional Information:
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          • Antibody Testing Data (1)
          DDK Antibody in Western Blot (WB)
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          DDK Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          DDK Antibody (TA150080) in WB

          Purified N-terminal DDK fused protein (Left lane) and lysate from HEK293 cells transfected with RC209482 containing C-terminal DDK tag (right lane) were separated with SDS-PAGE gel and immunoblotted with Goat anti-DDK tag polyclonal antibody (TA150080, 1:500). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          DDK Antibody in Western Blot (WB)

          Product Details

          TA150080

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-500-1:2,000
          -
          Product Specifications

          Species Reactivity

          Tag

          Host/Isotype

          Goat / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide conjugated to KLH

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5-1 mg/mL

          Storage buffer

          Whole serum

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Synthetic peptide sequence is DYKDDDDK

          Target Information

          DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

          View more View less

          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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