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pcDNA™5/TO Mammalian Expression Vector - FAQs

View additional product information for pcDNA™5/TO Mammalian Expression Vector - FAQs (V103320)

2 product FAQs found

What dose of tetracycline or doxycycline inducer should I administer to transgenic mice harboring the T-REx system or other tet-inducible system?

We have not performed such experiments at Thermo Fisher Scientific, but external references indicate that you may use doxycycline or tetracycline at 200µg/ml in drinking water. Add 5% sucrose to the drinking water - the sugar makes the water palatable for the mice.

Specific citations for these methods: Immunity 2000, 12(5):459-469; PNAS 1996, 93:10933-10938; Eur J Biochem 1999, 260(2):533-539.

What concentration of doxycycline should I use for induction in the T-REx system?

The recommended concentration of doxycycline for induction in the T-REx system can vary from 10ng/ml -1µg/ml. For best results, you should pre-test the dose response for your specific cell line.