pGAPZ A, B, & C Pichia pastoris Expression Vectors

Catalog number: V20020

Invitrogen™  Related applications: Protein Expression

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pGAPZ A, B, & C and pGAPZ A, B, & C are expression vectors designed for high-level, constitutive expression in Pichia pastoris. pGAPZ and pGAPZ were created by replacing the methanol-regulated AOX1 promoter with the constitutive, glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter in the backbone of the pPICZ vectors. Although the yield
of any protein constitutively expressed in the Pichia system is dependent on the toxicity of the protein to yeast, constitutive expression under the control of pGAPZ or pGAPZ vectors can sometimes produce greater yields than inducible expression (1).
For Research Use Only. Not for use in diagnostic procedures.



Promoter: GAP
Vector Type: pGAP
Product Size: 20 µg
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x), c-Myc Epitope Tag
Antibiotic Resistance (Bacterial): Zeocin™ (ZeoR)

Contents & storage

All vectors are supercoiled and are provided lyophilized (store at -20°C). A lacZ positive expression control strain is provided with the pGAPZ vector. Store strains at +4°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols