pTrcHis A, B, & C Bacterial Expression Vectors

Catalog number: V36020

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Our pTrcHis A, B, & C vectors are designed to offer enhanced translation initiation and high-level expression in E. coli. These vectors feature:

• High-level regulated transcription from the trc promoter
• Enhanced translation efficiency of eukaryotic genes in E. coli
• The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain

This particular vector offers:

• N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-HisG antibody
• N-terminal Xpress™ epitope for easy detection with an anti-Xpress™ antibody
• Enterokinase cleavage site for removal of fusion tag

For C-terminal polyhistidine tag and c-myc epitope, please see our pTrcHis2 A, B, & C Vector.
For Research Use Only. Not for use in diagnostic procedures.



Vector Type: pTrc
Cloning Method: Restriction Enzyme ⁄ MCS
Promoter: Trc and lacO
Protein Tag or Fusion: Thioredoxin
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Product Size: 20 µg
Inducing Agent: IPTG
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible

Contents & storage

All vectors are provided in suspension. A TOP10 E. coli stab and positive expression control is also provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.


Manuals & protocols

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