pAd/PL-DEST™ Gateway® Vector Kit

Catalog number: V49420

Invitrogen™  Related applications: Antibody & Cell Culture Production | Gateway Cloning | Protein Expression | Viral Vaccine Production

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The pAd⁄PL-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄PL-DEST® vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Allows gene of interest to be controlled by a promoter of choice
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Key Features
• Gateway® Technology for efficient and rapid cloning
• Promoterless vector that allows gene of interest to be controlled by a promoter of choice
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Ampicillin selection marker

Kit includes
• pAd⁄PL-DEST™ Vector
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.



Promoter: None (Promoterless)
Key Function: Viral Expression, Clone your own Promoter
Protein Tag or Fusion: Untagged
Delivery Method: Adenoviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): None
Vector Type: pDEST
Product Size: 6 µg
Constitutive or Inducible System: Constitutive

Contents & storage

• pAd⁄PL-DEST™ Vector 150 ng⁄µl in TEBuffer, pH 8.0. 40 µl (Store at -20°C)
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 µg⁄µl in TE Buffer, pH 8.0. 10 µl (Store at -20°C)


Manuals & protocols