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pLenti6/V5-DEST™ Gateway® Vector

Catalog number: V49610

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

The pLenti6⁄V5-DEST® Gateway® Vector is a Gateway®-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells

Key Features
• Flexible and versatile Gateway® recombination cloning technology
• Constitutive high expression with CMV promoter
• Blasticidin selection marker for stable selection
• C terminal V5 tag for quick detection

Kit includes
• pLenti6⁄V5-DEST™ Gateway® Vector
• One Shot® Stbl3™ Chemically Competent E. coli (C7373-03)

Related SKUs
• pLenti6⁄UbC⁄V5-DEST™ Gateway® Vector (V49910)
• pLenti4⁄V5-DEST™ Gateway® Vector (V49810)
• pLenti6⁄V5 Directional TOPO® Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO® Expression Kit (K495000)
• ViraPower™ Lentiviral Gateway® Expression Kit (K4960-00)
• ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (K5330-00)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pDEST, pLenti
Constitutive or Inducible System: Constitutive
Product Size: 6 µg

Contents & storage

ViraPower™ Lentiviral Gateway® Vectors, store at –20°C includes:

pLenti6⁄V5-DEST™: 6 µg (40 µl of 150 ng⁄µl vector in 10 mM
Tris-HCl, 1mM EDTA, pH 8.0)

pLenti6⁄V5-GW⁄lacZ control: 10 µg
(20 µl of 0.5 µg⁄µl vector in 10 mM
Tris-HCl, 1mM EDTA, pH 8.0)

One Shot® Stbl3™ Chemically Competent E. coli store at –80°C

Documents

Manuals & protocols

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