pLenti6/UbC/V5-DEST™ Gateway® Vector

Catalog number: V49910

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

Invitrogen™'s pLenti6⁄UbC⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ UbC Lentiviral Gateway® Expression Kit (catalog K4990-00).

The pLenti6⁄UbC⁄V5-DEST™ Gateway® Vector is a Gateway®-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway® recombination cloning technology

Key Features
• UbC promoter
• V5 epitope tag at C terminus
• Blasticidin selection

Kit includes
• A lacZ vector as a positive control, pLenti6⁄UbC⁄V5-GW⁄lacZ
• Stbl3™ competent cells

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: UbC
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pDEST, pLenti
Constitutive or Inducible System: Constitutive
Product Size: 6 µg

Contents & storage

Vector Kit (Box 1)
pLenti6⁄UbC⁄V5-DEST™:6 µg, store at -20°C
pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector: 10 ug, store at -20°C

One Shot® Stbl3™ Chemically Competent E. coli(Box 2)
Stbl3™ Cells: 21 x 50 µL, store at -80°C
pUC19 control DNA (10 pg⁄µL): 50 µL, store at -80°C
S.O.C. Medium: 6 mL, store at -80°C

Documents

Manuals & protocols