pLenti6.3/V5-DEST™ Gateway® Vector Kit

Catalog number: V53306

Invitrogen™  Related applications: Protein Expression

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Description

Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00).

The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway® recombination cloning technology

Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection

Kit includes
• A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ
• Stbl3™ competent cells

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pDEST, pLenti
Constitutive or Inducible System: Constitutive
Product Size: 1 kit
Selection Marker Promoter: SV40 Promoter

Contents & storage

Vector Kit (Box 1)
pLenti6.3⁄V5-DEST™ (150 ng⁄µL): 6 µg, store at -20°C
pLenti6.3⁄V5-GW⁄lacZ Control vector (500 ng⁄µL): 10 µg, store at -20°C

One Shot® Stbl3™ Chemically Competent E. coli (Box 2)
Stbl3™ Cells: 21 x 50 µL, store at -80°C
pUC19 control DNA (10 pg⁄µL): 50 µL, store at -80°C
S.O.C. Medium: 6 mL, store at -80°C

Documents

Manuals & protocols