pFRT/lacZeo and pFRT/lacZeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a:•Read more
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Catalog number V601520
Price (USD)/ 20 µg
732.00
-
Add to cart
Price (USD)/ 20 µg
732.00
Add to cart
pFRT/lacZeo Vector
Catalog numberV601520
Price (USD)/ 20 µg
732.00
-
Add to cart
pFRT/lacZeo and pFRT/lacZeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a:
• FRT site for subsequent targeted integration of a Flp-In™ expression vector Z-Zeocin™ fusion for selection of stable integrants with Zeocin™
These vectors differ in the SV40 promoter that drives expression of the lacZ-Zeocin™ fusion. pFRT/lacZeo2 contains a truncated version of the SV40 promoter. Use of this vector facilitates the isolation of clones that have integrated the vector near enhancer elements in the genome. Selection of stable clones using pFRT/lacZeo2 may allow easier isolation of clones that express a gene of interest at higher levels.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterSV40
Product TypeFlp-In System Expression Vector
Reporter GeneBeta-Gal (lacZ)
Selection Agent (Eukaryotic)Zeocin™
Protein TagUntagged
Quantity20 μg
VectorpFRT
Product LineFlp-In™
For Use With (Application)Targeted Integration
Unit Size20 µg
Contents & Storage
20 μg of vector, either pFRT/lacZeo or pFRT/lacZeo2, is provided supercoiled and lyophilized. Store at -20°C once resuspended. Vectors are guaranteed stable for 6 months when properly stored.