pIB/V5-His Vector Kit

Catalog number:  V802001

 Related applications:

Protein Expression

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The InsectSelect™ BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells:

• The OpIE2 promoter for constitutive expression
• The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.



Cloning Method: Restriction Enzyme ⁄ MCS
Product Line: InsectSelect™
Product Size: 1 kit
Promoter: OplE2
Protein Tag Position (to your gene): C-terminal
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Vector Type: pIB

Contents & storage

The InsectSelect pIB/V5-His Vector Kit includes 20 µg each of supercoiled lyophilized pIB/V5-His and pIB/V5-CAT vectors, and 2 µg lyophilized OplE2 Forward and Reverse sequencing primers.

Store all reagent at -20°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols