pYES2/NT and pYES2/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Both vectors offer the URA3 gene for selection in yeast. pYES2/NT features an N-terminal Xpress™ epitope for detection with Invitrogen's Anti-Xpress™ Antibody and a polyhistidine (6xHis) tag for purification with nickel-chelating resin. pYES2/CT contains a C-terminal V5 epitope for detection and a polyhistidine (6xHis) tag for purification.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
GAL1
Product Type
Yeast Expression Vector
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
Restriction Enzyme/MCS
Vector
pYES
Product Line
YES™
Contents & Storage
Vectors are supplied supercoiled and lyophilized. pYES2/NT is provided in three reading frames. Both pYES/NT and pYES/CT include a lacZ expression control vector and an INVSc1 yeast stab. Store vectors at -20°C. Store the stab at +4°C. All reagents are guaranteed stable for 6 months when properly stored.